GSM8078802: Amphioxus_21hpf_ATACseq rep1; Branchiostoma lanceolatum; ... - SRA

SRX23619397: GSM8078802: Amphioxus_21hpf_ATACseq rep1; Branchiostoma lanceolatum; ATAC-seq
1 ILLUMINA (NextSeq 500) run: 28.2M spots, 2.8G bases, 1.3Gb downloads

External Id: GSM8078802_r1

Submitted by: Sebe-Pedros lab, Systems Biology, Centre for Genomic Regulation

Study: An amphioxus neurula stage cell atlas supports a complex scenario for the emergence of vertebrate head mesoderm

PRJNA1076257 • SRP489656 • All experiments • All runs

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We performed single-cell transcriptome analysis (using MARS-seq) and accessible chromatin profiling (ATAC-seq) in Branchiostoma lanceolatum embryos (21 hours post-fertilization) Overall design: Embryos were dissociated and pooled before sorting single-cells for scRNAseq.

Sample: Amphioxus_21hpf_ATACseq rep1

SAMN39942007 • SRS20459713 • All experiments • All runs

Organism: Branchiostoma lanceolatum

Library:

Name: GSM8078802

Instrument: NextSeq 500

Strategy: ATAC-seq

Source: GENOMIC

Selection: other

Layout: PAIRED

Construction protocol: ATAC-seq was perform as detailed in the OmniATAC protocol (doi.org/10.1038/nmeth.4396). Around 50,000 cells were used for each tagmentation reaction. 21hpf embyos (19C) were dissociated and pooled before sorting single-cells for scRNAseq or ATAC-seq experiments.

Runs: 1 run, 28.2M spots, 2.8G bases, 1.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR27962982	28,153,531	2.8G	1.3Gb	2024-04-10

ID:: 31895118

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